Univ. of Murcia (UM)
The Toxicology and Forensic Veterinary Group has been constituted in 1998 and has 6 staff members and PhD students. The Group is mainly devoted to basic and applied research in Ecotoxicology and Environmental Toxicology of persistent environmental contaminants (heavy metals and organochlorine compounds) using several monitoring species such as mammals, birds, fishes, etc. These species are used to evaluate the exposure to contaminants in order to obtain useful information to both, human and environment risk assessment.
The Group is developing new processes to evaluate toxic effects using alternative in vitro methods and innovative methodologies. It has demonstrated experience in toxicity, ecotoxicity and cytotoxicity tests, mainly in aspects related with biochemical and morphological alterations of exposed cells to environmental contaminants. During the last two years we have being developing studies of cytotoxicity of antibiotics using established and primary cells.
The models and species used by our Group in toxicity, ecotoxicity and cytotoxicity tests include: rat, mouse, rabbit, Japanese quail and mallard in toxicity tests; Daphnia magna, Dicentrarchus labrax, Cymodocea nodosa, RTG-2, BF-2 in ecotoxicity tests; BGM, VERO, PK-15, CHO-K1, LLCPK1, A-Mel-3, CHP-212, avian erythrocytes, chromaffin cells in cytotoxicity tests.
The techniques and methodologies commonly applied in the tests include: Neutral red assay, MTT assay, Flow Cytometry, Cell cycle, optical, confocal and electronic microscopy (transmission and scanning), biochemical markers of oxidative stress, chemical-toxicological analysis, etc.